Plasmid_Backbone

Part:BBa_K4216043:Design

Designed by: Yves Loiseau--Marchand   Group: iGEM22_Paris_Bettencourt   (2022-10-11)


pACYDuet_Bettencourt


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2177
    Illegal SpeI site found at 2409
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 1336
    Illegal NheI site found at 2434
    Illegal NheI site found at 2457
    Illegal SpeI site found at 2409
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2177
    Illegal SpeI site found at 2409
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2177
    Illegal SpeI site found at 2409
    Illegal AgeI site found at 150
    Illegal AgeI site found at 1422
    Illegal AgeI site found at 1745
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3425
    Illegal BsaI.rc site found at 2390


Design Notes

N/A


Source

Synthetic DNA and pACYDuet Plasmid

References